Rubber dam isolation is essential for the safety and efficiency of treatment using microscopes in which precise procedures are carried out, since it allows concentration on the operating field. When isolating multiple teeth in the molar region, problems may be encountered, such as wrinkling of the rubber dam, exposure of the gum, or unbalanced positioning of the sheet too far in one direction. However, there do not appear to be any papers that address such problems. The objective of the present study was to examine the sideways positional relationship of the punched holes in the rubber dam, the interval between punched holes, and the range of isolation, in order to consider methods for favorable rubber dam isolation. An oral cavity cover and a model jaw were fitted to a simple manikin. The teeth for isolation were #23–#27. Five types of rubber dam templates were used for the study. Conclusion: It was found that, when a template is used for isolation of multiple teeth in the molar region, appropriate rubber dam isolation can be obtained by shifting the punching holes on the template 10 mm from the center. In addition, with the model used in the study, as well as setting the isolation range at #11–#27, the most appropriate rubber dam isolation was carried out by placing a clamp over the rubber sheet at #15, setting the interdental intervals at #23–#26, the same as on the template by Roeko, and setting the interval of #26–#27 at 8 mm. The results suggested that favorable isolation of multiple teeth in the molar region can be achieved by shifting the punching sites about 10 mm in the direction away from the side to be isolated, giving a more inner curve than the original curve of the dental arch, and setting the interdental interval slightly short.
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