In dental treatment, water is applied at room temperature during treatment to protect the cells from nociceptive stimulation by heat. In this study, the purpose was to confirm the use of bicarbonate Ringer's solution as an alternative to water in dental treatment. The main cells to be protected in dental treatment were preserved in water, saline and bicarbonate Ringer's solution for 60 minutes. Three different temperature conditions were used for storage. The results showed that for all liquid storage conditions, the temperature conditions closer to body temperature resulted in less cell death and higher mitochondrial activity. For the nutrient conditions, the closer the conditions were to body temperature, the less cell death and the higher the mitochondrial activity tended to be. The ability to survive more cells and maintain higher mitochondrial activity suggests the usefulness of bicarbonate Ringer's solution for dental treatment.