Nosocomial infections that endanger patients are mainly transmitted through the hands of healthcare workers. At Tokyo University of Technology, 48 students (96 palms) underwent practical training in accordance with WHO hand washing guidelines. As a result, only 33 hands passed the adenosine triphosphate (ATP) test. Therefore, to optimize handwashing instruction, handwashing behaviors and cleaning results were compared. First, 41 subjects (82 palms) were measured for wrist acceleration during handwashing and ATP values of their hands before and after handwashing. The ATP values before hand washing ranged from approximately 6,000 to 180,000 RLU, making it difficult to determine whether the hand washing behavior was “satisfactory” or “unsatisfactory”. Nine students (18 palms) were then ultrasonically cleaned, and ATP lotion was applied to keep ATP concentrations in a certain range before hand washing. Analysis of ATP removal rates and hand movements revealed that routine ATP concentration control was the most important factor, followed by lateral hand movements, keeping hands horizontal during hand washing, and the results will be utilized in future handwashing instruction.
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