The vitamin requirements of the mycelial phase and the yeast phase of 12 strains of S. schenckii were compared. These strains were isolated from human cases of sporotrichosis. The following basal medium was used for the mycelial and the yeast phase; 10 g glucose, 3.3 g Na₂HPO₄, 1.1 g KH₂PO₄, 0.25 g MgSO₄ 7H₂O, 0.25 g NaCl, 12 g agar (OXOID), and 1000 ml distilled water. Aspartic acid (2 g/L) was added in the medium as the source of nitrogen. The pH was adjusted to 7.4 with 1 N NaOH. The medium was autoclaved at 121°C for 15 mim.
In the experiments of the vitamin requirements, the following 9 vitamins were employed; d-biotin, thiamine hydrochloride, calcium pantothenate, pyridoxine hydrochloride, folic acid, riboflavin, nicotinic acid, p-aminobenzoic acid and inositol. Each vitamin was sterilized by filtration.
In order to learn whether any single growth factor was necessary for the growth process in the mycelial phase or the yeast phase, a series of slants which omitted with a different one of the 9 growth factors from each slants were prepared. At the same times, another series which were added each one of the 9 growth factors were prepared. Cultures of the yeast phase were incubated at 37°C for 10 days and ones of the mycelial phase at 25°Cfor 10 days.
The result obtained was as follows; thiamine hydrochloride was necessary for the growth of the mycelial phase and the yeast phase. On the other hand, nicotinic acid and inositol were not necessary for the development of the yeast phase. Also the other 8 vitamins except thiamine hydrochloride was not necessary for the mycelial phase.

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The relationship between the types of eruption and the parasitic forms of Trichophyton rubrum was studied.
Parasitic forms were observed by the direct microscopic examination. Materials were obtained from the patients' scraped specimen of erythema, papule, scale or the nail and were examined in 20% potassium hydroxide. The roof of vesicle or pustule was cut off by scissors and was examined in 20% potassium hydroxide.
According to the formation of arthrospore, the parasitic forms of Trichophyton rubrum were classified into three types (Type A, B and C). Type A : Hyphae without remarkable septum and with septa, if found, at intervals of more than 5μ.
Type B : Hyphae with septa at intervals of less than 5μ and the width of hyphae less than 5μ.
Type C : Individual arthrospore detached from hyphae, swelled and shaped spherically (some of them occasionally germinated). Its diameter was more than 5μ.
The relationship between the types of eruption and the parasitic forms was as follows : The lesions of erythema, papule and scale were occupied by 81.6% type A, 18.4% type B and none type C. The lesions of vesicule were occupied by 53.3% type A, 46.7% type B and non type C, while the lesions of pustule by 33.3% type B, 66.7% type C and none type A. The white-macerated-soggy lesions carried 7.7% type A, 76.9% type B and 15.4% type C. While tinea unguium 50.0 % type A, 10.0% type B and 40.0% typeC.
The lesions that appear erythema, papule and scale are dry, whereas the lesions that appear vesicle, pustule, and white-macerated-soggy condition are moist. The hyphae in these moist lesions contact immediately with serous, mucinous, fibrinous, purulent or sanguinous exudate. Regarding to the parasitic forms in the lesions, type B and C were much frequently observed in the moist lesions than in the dry lesions. The swollen spherical cells (type C) were found in 8 of 79 cases, namely 2 in the lesions of pustule, and 2 in the white-macerated-soggy condition, and 4 in tinea unguium.

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