A perithecium like organ found in a crust specimen from a patient with sporotrichosis was examined by the direct fluorescent antibody technique.
The specimen previously stained with hematoxylin was immersed in xylol to remove a coverslip and Canada balsam, then passed through alcohols to staining buffer. The staining procedure was carried out by covering the specimen with the fluorescent antibody of S. schenckii (Sp-56) and allowing it to stand overnight at room temperature in a moist chamber. Then, after being treated with the usual procedures, it was observed under a fluorescence microscope FM-200B (Chiyoda Kogaku).
As a result specific fluorescence was observed on hyphae and conidia forming the perithecium like organ.

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