In the treatment of infected root canals, we must remove both organic and inorganic substances, such as infecteddentin, residual pulp tissue, and bacteria. In the clinic, mechanical cleaning and chemical irrigation of root canals are importantprocesses to ensure healing. However, thorough cleaning of the root canal system is difficult to achieve due to lateral branches, apical ramifications, and strictured root canals such as fins and isthmuses. Today, fine and flexible laser fibers have beendeveloped and applied in the clinic. The aims of this study were to investigate the effects of diode laser irradiation on Staphylococcus aureus, and to evaluate the temperature elevation on the root surfaces during diode laser irradiation in the root canal.
In Experiment 1, extracted human teeth with single roots were used. After removing the crowns, the root canals wereenlarged to #35 1 mm short of the anatomical apex. After root canal irrigation with NaClO and EDTA, the samples were furtherultrasonically irrigated for five minutes. When the laser fiber or the plugger of the heat carrier was irradiated or activatedupon being withdrawn from the apex at a speed of 1 mm/s for ten seconds, the maximum temperature elevation on the rootsurfaces was measured. Laser irradiation parameters chosen were 30mJ 66pps, 45mJ 66pps, 60mJ 66pps, 45mJ 100pps, and 60mJ 100pps, and the tip of the heat carrier was set at 200°. Additionally, the surfaces of the root canal after irradiation wereobserved under a digital microscope and a scanning electron microscope (SEM). In comparison with the heat carrier, a significantly lower temperature elevation was observed for laser irradiation at 30mJ 66pps and 45mJ 66pps. In the digital microscopy and SEM observation, melted dentin was observed without carbonization or defects after lasing at 45mJ 66pps.In Experiment 2, fifteen roots of intact bovine incisors were used. After canal irrigation with NaCIO and EDTA, the sampleswere further ultrasonically irrigated for five minutes. The root surfaces were coated with nail enamel, and the samples weresterilized by an autoclave. Each root canal was filled with 100-E1 of S. aureus (2.0×10
8 CFU/ml) culture medium and incubatedat 37° for 24 h. Then, the samples were divided into three groups. In group 1, the culture medium in the root canals wasirradiated by diode laser (45mJ 66pps) under an up-and-down motion between the apical and cervical portion with an opticalfiber (diameter=400, μm) for 10 seconds. In group 2, the plugger of the heat carrier was activated as in group 1. In group 3, the laser fiber was moved in the canal without irradiation. After the above procedures, bacterial samples were taken fromeach root canal, and 50μ of each sample was incubated aerobically on Soy Sonic Broth at 37° 24 h. The numbers of colonieson the medium were counted, and colony-forming-units (CFUs) /ml were calculated. CFUs of groups 1, 2 and 3 were 1.2×1011CFUs/ml, 7.6×1011 CFUs/ml, and 5.4×1012 CFUs/ml, respectively. Statistical analysis revealed that CFUs in groups 1 and 2were more reduced than in group 3 and that those in group 1 were more reduced than in group 2 (P< 0.05). These resultssuggest that diode laser irradiation could be used for root canal disinfection safely and effectively.
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