We examined the changes in electromyographic reaction time (EMG-RT) with increases in the number of auditory stimuli that were administered at regular intervals, and confirmed that the factor of forecast influenced EMG-RT when stimulation was carried out at regular intervals. In the present study, we examined whether the factor of forecast influences EMG-RT on the basis of the difference in interstimulus interval (ISI). Ten healthy subjects (eight males, two females; mean age 26.5 ± 5.6 years) participated in this study. The characteristics of the auditory stimulus study were a stimulus frequency of 900 Hz with 15 consecutive stimuli per trial. The subjects were requested to raise their right ankle in response to each auditory stimulus. A total of three different ISIs (900, 2,700, and 4,500 ms) were used in this study. The influence of the increase in the number of stimuli on EMG-RT was examined. At each ISI, EMG-RT showed a tendency to decrease at the 2
nd and 3
rd stimulus compared with 1
st and 2
nd stimulus. At an ISI of 900 ms (n=6), EMG-RT observed at the 8
th-15
th stimuli were significantly decreased compared with that observed at the 1
st stimulus, whereas EMG-RT observed at the 2
nd-7
th stimuli were not. At an ISI of 2,700 ms, EMG-RT observed at the 3
rd-15
th stimuli were significantly decreased compared with that at the 1
st stimulus. At an ISI of 4,500 ms, EMG-RT observed at the 3
rd-6
th and 8
th stimuli were significantly decreased compared with that at the 1
st stimulus, but EMG-RT at 7
th and 9
th, 15
th stimuli were not. Four subjects, whose muscle activities were recorded before the input of the auditory stimulus, were excluded from the analysis at 900-ms ISI. In the remaining six subjects, forecast was initiated following stimulus by recognition of ISI at the 3
rd stimulus and was adjusted to the frequency during the 3
rd-7
th stimuli. We consider that the results at the 8
th or subsequent stimuli may be affected by the factor or automation and the results at 2,700-ms ISI have been affected by continuation of attention to the stimulation as well as the forecast of the following stimulation. At the 4,500-ms ISI, the forecast of the following stimulation is difficult because recognition of periodic rhythm is difficult; therefore, attention should be paid to the ISI. We hypothesize that the attention continued up to the 8
th stimulus and attention at the 8
th or subsequent stimuli was diverted, which may have affected the results.
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