Journal of Medical and Dental Sciences
Online ISSN : 2185-9132
Print ISSN : 1342-8810
ISSN-L : 1342-8810
Volume 44, Issue 3
Displaying 1-2 of 2 articles from this issue
  • Shigeru Oda, Atsuhiro Kinoshita, Tatsuhiro Higuchi, Toshio Shizuya, Is ...
    1997 Volume 44 Issue 3 Pages 53-62
    Published: 1997
    Released on J-STAGE: July 31, 2017
    JOURNAL OPEN ACCESS
    We investigated ectopic bone formation by biphasic calcium phosphate (BCP) combined with recombinant human bone morphogenetic protein-2 (rhBMP-2) in the rat dorsum. Under reduced pressure, rhBMP-2 was adsorbed onto BCP, which consisted of 80% β-tricalcium phosphate and 20% hydroxyapatite uniformly distributed in granules. Twenty Wistar rats were separated into 4 groups consisting of 5 animals each dosed with 2, 10, and 50 µg/700 µl of rhBMP-2 and a control group (BCP only). Pieces of the BCP-BMP complex or only BCP were implanted under the dorsal skin of the rats. Histological sections were examined three weeks later. New bone was formed in all rats given 50 µg doses, but not in the 2 µg and control groups. These results indicated that BCP combined with rhBMP-2 induced ectopic bone formation without additional carriers. Therefore, BCP granules alone can function as carriers for rhBMP-2 to induce bone formation.
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  • Izumi Asahina, Masatoshi Watanabe, Norio Sakurai, Masaji Mori, Shoji E ...
    1997 Volume 44 Issue 3 Pages 63-70
    Published: 1997
    Released on J-STAGE: July 31, 2017
    JOURNAL OPEN ACCESS
    The purpose of this study was to evaluate a hydroxyapatite (HA)-collagen (Col)-bone morphogenetic protein (BMP) composite as an osteoinductive bone substitute. Partially-purified BMP from bovine bone was mechanically mixed with highly purified type I collagen from calf dermis and then subsequently mixed with pure synthetic hydroxyapatite granules or block. The HA-Col-BMP composite, or the HA-Col composite as a control, was implanted in a surgically-induced mandible bone defect (6×7×10 mm) in an adult Japanese monkey. The mandible was excised three months after implantation and studied histologically. The BMP-containing implant induced much more new bone than the control implant in all experimental animals of each group, with either HA granules or HA block. Newly formed bone was attached tightly to HA and infiltrated deeply into the pores of the HA of the BMP-containing implant, while fibrous tissue existed between the host bone and HA in the control implant. Thus, we conclude that an HA-Col-BMP composite could be a superior biomaterial for a bone substitute.
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