We analyzed the periodic vasomotion of arterioles in the mesentery of anesthetized rabbits by means of simultaneous multiple-site measurement of inside diameters. For this purpose we newly developed a frame-by-frame diameter detection technique using a microcomputer-assisted laser video disk recorder and a video image analysis system.
We simultaneously extracted intensity profiles of different sampling sites across an arteriole from original video recordings and reconstructed sets of time series of intensity profiles by scanning consecutive video frames recorded on a video disk recorder. Thereafter, we computed temporal changes of inside diameter from the sets of intensity data.
Frequency analysis for temporal changes of inside diameter showed amplitude, amplitude ratio and frequency of vasomotion to be 1.36 ± 0.23 μm (mean ± S.E., n = 14), 7.02 ± 0.97% and 9.0 ± 1.9 cycle/min, respectively. Mean inside diameter of the arterioles was 20.0 ± 2.0 p m. A negative correlation was found between amplitude ratio and frequency of vasomotion ( r =-0.654, p<0.01).
We evaluated propagation velocity ( 0.11 ± 0.02 mm/s ) of vasomotion of the arteriole by cross-correlation method. We found a positive correlation between vasomotion frequency and propagation velocity of vasomotion (r= 0.667, p<0.01) and a negative correlation between amplitude ratio and propagation velocity of vasomotion (r =-0.649, p<0.01).
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