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Ichirou MOTOKI, Takashi YOKOGAWA, Tsunemi HASEGAWA, Kimitsuna WATANABE ...
1993 Volume 5 Issue 4 Pages
85-90
Published: February 15, 1994
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Hydroxyl radical, generated by reduction of hydrogen peroxide by Fe (II)-EDTA, was used to investigate the higher-order structure of yeast tRNA
Tyr and its chimeric mutants in aqueous solution. Exposure of free tRNA
Tyr to this reagent gave cleavage patterns consistent with the three-dimensional structure of yeast tRNA
Phe established by X-ray crystallography. This probing method was applied to detect the base-mismatches in the anticodon stem region of a chimeric tRNA
Tyr mutant, which was constructed by ligating the 5′-half fragment of
T. utilis tRNA
Tyr and the 3′-half fragment of baker's yeast tRNA
Tyr with T4 RNA ligase. The result of this analysis clearly showed that this probing method is sensitive enough to detect even a minute variation of the secondary structure of RNA molecules.
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Tsunemi HASEGAWA, Katsutoshi MURAO, Kazuya NISHIKAWA, Hisayuki ISHIKUR ...
1993 Volume 5 Issue 4 Pages
91-94
Published: February 15, 1994
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The coding properties of 5-methoxyuridine in the wobble position of threonine and valine tRNAs from
Bacillus subtilis were investigated by the technique of aminoacyl-tRNA binding to ribosomes. 5-Methoxyuridine is recognized by A, G, and fairly well by U of the third letter of colons like uridine-5-oxyacetic acid in some tRNAs from
Escherichia coli.
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Nobukazu NAMEKI, Kazuya NISHIKAWA, Tsunemi HASEGAWA
1993 Volume 5 Issue 4 Pages
95-98
Published: February 15, 1994
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To study the evolution of the tRNA recognition mechanism, we constructed variants of baker's yeast tRNA
Thr transcripts using an
in vitro transcription system and compared the recognition elements of yeast tRNA
Thr with those of
Escherichia coli tRNA
Thr. In yeast tRNA
Thr, the second and third positions of the anticodon, G35U36, are major recognition sites, whereas the discriminator base A73 is not involved in aminoacylation. These are the same as in
E. coli tRNA
Thr. These findings indicate the similarity of the recognition system by the cognate threonyl-tRNA synthetase functions in
E. coli and yeast.
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Masahide TAKADA-HIDAI
1993 Volume 5 Issue 4 Pages
99-104
Published: February 15, 1994
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We explain examples of the scientific studies which are planned to be undertaken with the SUBARU High Dispersion Spectrograph (HDS). The current specifications of the HDS are briefly described.
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Suehiko KOYAMA, Kyoji TACHIKAWA
1993 Volume 5 Issue 4 Pages
105-106
Published: February 15, 1994
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The Cu-Si alloy has been proposed as a new matrix material for filamentary Nb-Ti wires in AC use. The Cu-Si alloy shows appropriate mechanical and electrical properties. The addition of Si to the Cu matrix prevents the formation of intermetallic compounds around the Nb-Ti filaments, Ultrafine multifilamentary Nb-Ti/Cu-3.5wt%Si composite wires have been successfully fabricated. The wires show excellent performances with respect to AC loss and critical current density.
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Yoshihiro NAGAKI, Tsuyoshi NAKAHARA, Kyoji TACHIKAWA
1993 Volume 5 Issue 4 Pages
107-108
Published: February 15, 1994
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Recent studies on the fabrication of Bi (Pb)-2223 high-Tc oxides are described. Ag-sheathed tapes have been made by a powder-in-tube technique using the diffusion reaction between a high melting point and a low melting point components. Critical current density of the tape has reached 4.2×10
4A/cm
2 at 77K and OT. The preparation of Ag-sheathed multilayered tapes are being performed through a new route.
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Hiroaki Komatsu, Masayosi Tada
1993 Volume 5 Issue 4 Pages
109
Published: February 15, 1994
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Tadaaki Amano, Kaoru Michiyama, Yasuhiko Nakajima, Nobutaka Suzuki, Hi ...
1993 Volume 5 Issue 4 Pages
110
Published: February 15, 1994
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Tadaaki Amano, Shingo Takehana, Tetsuya Masuda, Toshiyuki Watatani, Sh ...
1993 Volume 5 Issue 4 Pages
111
Published: February 15, 1994
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Hideo Hinowa, Hiroki Yamashita, Tsunenori Suzuki
1993 Volume 5 Issue 4 Pages
112
Published: February 15, 1994
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Mitsuo Mutuura
1993 Volume 5 Issue 4 Pages
113
Published: February 15, 1994
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Hiroshi SUGIMOTO, Takao HONMA, Tadayoshi YACHI
1993 Volume 5 Issue 4 Pages
114
Published: February 15, 1994
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Kazuhiro Yoshida, Takayoshi Yachi, Takatoshi Miyasaka
1993 Volume 5 Issue 4 Pages
115
Published: February 15, 1994
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Mitsuhiro WADA, Atsushi YAMAMOTO, Akiko ICHIKAWA, Masanori TAKI, Masar ...
1993 Volume 5 Issue 4 Pages
116
Published: February 15, 1994
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Akiko ICHIKAWA, Mitsuhiro WADA, Atsushi YAMAMOTO, Masanori TAKI, Masar ...
1993 Volume 5 Issue 4 Pages
117
Published: February 15, 1994
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Satoshi SUZUKI, Takehisa SHIBUYA, Shuichi KAWABATA, Hideshi YOKOTA
1993 Volume 5 Issue 4 Pages
118
Published: February 15, 1994
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Kazuki Aoyama, Yasunari Watanabe, Kazuteru Tashiro, Hirohisa Uchida
1993 Volume 5 Issue 4 Pages
119
Published: February 15, 1994
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Iichiro TOJO, Hiromi SUZUKI, Sumio KISE, Atsushi NAGAHAMA, Hirohisa UC ...
1993 Volume 5 Issue 4 Pages
120
Published: February 15, 1994
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Satoru Seki, Shoji Seta, Yasuo Naragaki, Hirohisa Uchida
1993 Volume 5 Issue 4 Pages
121
Published: February 15, 1994
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Masakazu SUGIURA, Nobuyuki TANAKA, Fumiya UEHARA
1993 Volume 5 Issue 4 Pages
122
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Takaaki Oku, Haru-Hisa Uchida
1993 Volume 5 Issue 4 Pages
123
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Kouji Ishizuka, Hiroko Yamamoto, Kazuya Oguri, Yoshitake Nishi
1993 Volume 5 Issue 4 Pages
124
Published: February 15, 1994
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Tetsuo AIDA, Kai SHIRAISHI, Yuichi KAWAGUCHI, Yoshitake NISHI
1993 Volume 5 Issue 4 Pages
125
Published: February 15, 1994
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Kaoru Usui, Sinichi Ichimura, Kazuya Oguri, Yoshitake Nishi
1993 Volume 5 Issue 4 Pages
126
Published: February 15, 1994
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Kai Shiraishi, Shigemi Watanabe, Takashi Ohwaki, Kazuya Oguri, Kazuo N ...
1993 Volume 5 Issue 4 Pages
127
Published: February 15, 1994
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Yuichi Kawaguchi, Tetsuo Aida, Kai Shiraishi, Yoshitake Nishi
1993 Volume 5 Issue 4 Pages
128
Published: February 15, 1994
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Akihiro Matsubara, Takeshi Noguchi, Kazuki Ishikawa, Akira Tonegawa, S ...
1993 Volume 5 Issue 4 Pages
129
Published: February 15, 1994
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Masanori Kouno, Shinichi Tanabe, Akira Tonegawa, Kazuo Takayama
1993 Volume 5 Issue 4 Pages
130
Published: February 15, 1994
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Atsushi KOBAYASHI, Hideki FUJITA, Hideshi YOKOTA, Teiji UCHIDA
1993 Volume 5 Issue 4 Pages
131
Published: February 15, 1994
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Kensuke Matushita, Moriaki Wakaki, Muneo Miyasaka
1993 Volume 5 Issue 4 Pages
132
Published: February 15, 1994
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Shin-itirou Toda, Moriaki Wakaki, Yuichi Komati
1993 Volume 5 Issue 4 Pages
133
Published: February 15, 1994
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Kazuyoku Tei, Hideshi Yokota
1993 Volume 5 Issue 4 Pages
134
Published: February 15, 1994
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Masayuki UENO, Shin-ichi OKUMURA, Masayuki YAMAZAKI
1993 Volume 5 Issue 4 Pages
135
Published: February 15, 1994
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Makoto Hikichi, Haru-Hisa Uchida
1993 Volume 5 Issue 4 Pages
136
Published: February 15, 1994
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Masakuni Matsumoto, Mitsumasa Uruga, Gou Yoshie, Akira Tonegawa, Kazuo ...
1993 Volume 5 Issue 4 Pages
137
Published: February 15, 1994
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Takasi Endoh, Manabu Takayanagi, Kuichi Murata, Mamoru Kawakami
1993 Volume 5 Issue 4 Pages
138-139
Published: February 15, 1994
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Tuyosi Sato, Takasi Endo, Mamoru Kawakami
1993 Volume 5 Issue 4 Pages
140-141
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Motoharu Watanabe
1993 Volume 5 Issue 4 Pages
f1-f4
Published: February 15, 1994
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Kazuo TAKAYAMA, Akira TONEGAWA
1993 Volume 5 Issue 4 Pages
f12-f15
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Katsumi ARASAKI
1993 Volume 5 Issue 4 Pages
f16-f20
Published: February 15, 1994
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