Yolk sac of chicken eggs infected with Rickettsia mooseri was cultivated, and the rickettsiae in the grown cells were observed with light- and electron microscopes.

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Affinity of the rickettsiae to tissue cells, especially the relation between rickettsiae and host cells has been studied.
Rickettsiae infected tissues of embryonated chicken eggs were cultivated, and observations were made on the growth cells. On the other hand, normal tissues of the same kinds were cultivated side to the infected tissues, and susceptibility of each tissue to the infection was examined
Epithelial cells were found to be more susceptible to the infection than fribroblasts.

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In the present paper the staining properties and chemical nature of some inclusion bodies (Fig. 1) found in the cytoplasm of leaf epidermal cells of wheat plants were investigated. Trypan blue does not stain nuclei, while stains X-bodies violet after fixing in Carnoy's fluids. When erythrosin was appied as a counterstain following the trypan blue, the nuclei were stained violet and the X-bodies red. Both erythrosin-me thylene blue and pyronin-methyl green have been proved effective for staining selectively the bodies. The Giemsa method described by BALD (1919) was also tested, obtaining good results.
Sections pretreated with trichloracetic acid (TCA), perchloric acid (PCA), or hydrochloric acid in order to remove nucleic acid were stained with trypan blue. The X-bodies were still stained as were those in the control sections indicating that the dye was staining protein in these bodies (Fig. 5). The TCA- or PCA-pretreated nuclei and X-bodies were not stained with pyronin-methyl green; while the HCI-pretreated nuclei stained light-blue with the same dye, and the same treated bodies remain unstained. These results seem to indicate the presence of ribonucleic acid (RNA) in the bodies.
The Millon reaction, biuret test, xanthoproteic test, azoreaetion, ninhydrin test, and Thomas' test for arginine have all proved to provide evidence of protein in the bodies, The Xbodies were alo stained with Sudan III proving the presence of lipoid. They were not stained in Feulgen reaction (Fig. 6). The crystal inclusions possessing the properties of protein were also found in the cytoplasm (Fig. 2, 4) and within the vacuole in the X-bodies (Fig. 3).

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The onset of eastern equine encephalomy elitis and western equine encephalomyelitis can be observed following the intratesticular inoculation of the viruses in mice. The virus content of each organ (blood, spleen, testis, brain and spinal cord) of the inoculated mice was observed periodically by means of intracerebral inoculation of mice.
The obtained results show that the viruses inoculated intratesticularly enter the blood circulation immediately and then propagate rapidly in the central nervous system. Following the virus multiplication in the central nervous system, the intratesticular multiplication was also recognized.

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Measles virus, which derived from patients and was attenuated by intratesticular passages through guinea pigs, was inoculated into susceptible children by subcutaneous injection or intranasal spraying, Clininical and immunological findings observed were as follows:
1) A mild fever as well as change apparently the Koplik spot were found only in one case of ten, but neither katarrhal signs such as cough, sneeze or conjuctivitis nor eruptions were evident.
2) Of the blood picture, specific findings were: Initial leucocytosis followed by the leucopenia, relative neutropoilia and lymphopenia, as well as slight monocytosis and eoslnopenia. These changes were similar to those induces by the natural infection of meales.
3) It was deemed likely that the meales virus muliplied and the neutralizing antibodies were produced in the bodies of inoculated humans.

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We tired to prevent measles with attenuated measles virus, which was obtained from patients and weakend by intratesticular passage through guinea pigs.
The immunization was made by intranasal spraying for susceptible children.
The results obtained were as follows:
Classification of response Number Control
With evidence 130 (33.8%) 156 (59.%)
typical 12
mild 81
minimal 31
no count 6
No evidence 256 (66.2%) 106 (40.5%)
with chance to be infected
from patients with measles 111
no chance to be infected
from patients with measles 85
not clear 60
total 365 262
A-significicant differece could be found between the results of the prevented and control groups.

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[in Japanese]

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