Red blood cells in cardiopulmonary devices are subjected to unphysiological range of physical parameters . It is well known that the damage to red blood cells has relationship with physical parameters . However, a mechanism of hemolysis caused by the relationship is not clarified . The purpose of this study is to investigate the relationship between physical parameters and hemolysis by in vitro experiments . Bovine blood mixed with an anticoagulant was subjected to physical parameters: shear rate(0, 500, 1500s
-1), pressure(0, ±200, ±400, ±600 mmHg)and temperature(21, 28, 35°C). In consequence of these experiments, significant hemolysis was not observed at a shear rate of 0 or 500s
-1. At a shear rate of 1500s
-1, pressure more than200mmHg or less than-200mmHg induced significant hemolysis. In contrast, no hemolysis was observed under the other experimental conditions. In conclusions, it was found that pressure became an influential parameter on hemolysis when the shear rate was high. In addition, it was considered that the effect of pressure on hemolysis depended on the level of shear rate.
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