The PCR product is detected using real time PCR-SSP system with the double-labeled fluorogenic probe in a short time without any additional operation. The HLA-B allele typing which seemed to be the most difficult in the class I alleles was studied with this system. Thirty-four samples which the HLA-B antigen types had already-known were measured by ABI PRISMTM 7700 sequence detector using a commercial SSP primer kit and seven kinds of TaqMan probes designed by ourselves. All samples were obtained excellent reactivities in performing PCR amplification of 35 cycles, and they showed the amplifications expected from the specificities of the primers. The ratio of the weakest positive and the strongest negative A Rn values in each sample was 6.18 in average. Consequently, the identifications of the HLA-B alleles were clear and easy. The allele types identified by our developed real time PCR-SSP HLA-B typing system were in accordance with the serological HLA-B antigen types of panel DNA. The process from DNA extraction to the HLA-B allele identification finished in about two hours and artificial errors could be mininized, as the operation from PCR amplification to detection of amplicon was automatic.