Actinomycetologica
Online ISSN : 1881-6371
Print ISSN : 0914-5818
ISSN-L : 0914-5818
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Volume 15, Issue 1
Displaying 1-4 of 4 articles from this issue
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Original Articles
  • Nocardia vinacea sp. nov.
    Naoko Kinoshita, Yoshiko Homma, Masayuki Igarashi, Souichi Ikeno, Mako ...
    2001 Volume 15 Issue 1 Pages 1-5
    Published: July 25, 2001
    Released on J-STAGE: March 01, 2008
    DOIhttps://doi.org/10.3209/saj.15_1
    JOURNAL FREE ACCESS
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    This paper reports taxonomical studies on a soil isolate, the strain MK703-102F1, which produces a new antimycobacterial antibiotic tubelactomicin. We propose that this strain should belong to a new species of the genus Nocardia with the new name Nocardia vinacea sp. nov., the type strain being MK703-102F1 (= JCM 10988 = IFO 16497). This new species was characterized by meso-diaminopimelic acid as a cell wall diamino acid, arabinose and galactose as whole-cell sugars, N-glycolated muramic acids in cell walls, type PII phospholipids, MK-8(H4) menaquinone, fatty acid components consisting of hexadecanoic (16:0), octadecenoic (18:1), 10-methyloctadecanoic (10Me-18:0) and hexadecenoic (16:1) acids, mycolic acids with 48–56 carbons, some physiological characteristics, 65 mol% G+C in DNA, and base sequence of 16S ribosomal RNA gene (16S rDNA).
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  • A Rapid and Simple Method Using the Detergent Triton X114 for Preparation of Outer Membrane Proteins from Bacteria Containing Mycolic Acids
    Tomoko Aizawa, Noriyuki Iwabuchi, Aki Kikuchi, Makoto Urai, Hirosi Anz ...
    2001 Volume 15 Issue 1 Pages 6-10
    Published: July 25, 2001
    Released on J-STAGE: March 01, 2008
    DOIhttps://doi.org/10.3209/saj.15_6
    JOURNAL FREE ACCESS
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    A rapid and simple method using the detergent Triton X114 to prepare outer membrane proteins from whole cells of bacteria containing mycolic acids, α-alkyl, β-hydroxyl fatty acids is described. SDS-PAGE analysis and subsequent N-terminal amino acid sequencing revealed that the proteins prepared from whole cells by this method were almost identical to outer membrane proteins prepared by the conventional cell-disruption and differential-centrifugation method. Therefore, this method is useful to prepare outer membrane proteins from whole cells of Mycolata, bacteria containing mycolic acids.
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SAJ Award Lecture
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