Actinomycetologica
Online ISSN : 1881-6371
Print ISSN : 0914-5818
ISSN-L : 0914-5818
Volume 15 , Issue 1
Showing 1-4 articles out of 4 articles from the selected issue
Original Articles
  • Naoko Kinoshita, Yoshiko Homma, Masayuki Igarashi, Souichi Ikeno, Mako ...
    2001 Volume 15 Issue 1 Pages 1-5
    Published: July 25, 2001
    Released: March 01, 2008
    JOURNALS FREE ACCESS
    This paper reports taxonomical studies on a soil isolate, the strain MK703-102F1, which produces a new antimycobacterial antibiotic tubelactomicin. We propose that this strain should belong to a new species of the genus Nocardia with the new name Nocardia vinacea sp. nov., the type strain being MK703-102F1 (= JCM 10988 = IFO 16497). This new species was characterized by meso-diaminopimelic acid as a cell wall diamino acid, arabinose and galactose as whole-cell sugars, N-glycolated muramic acids in cell walls, type PII phospholipids, MK-8(H4) menaquinone, fatty acid components consisting of hexadecanoic (16:0), octadecenoic (18:1), 10-methyloctadecanoic (10Me-18:0) and hexadecenoic (16:1) acids, mycolic acids with 48–56 carbons, some physiological characteristics, 65 mol% G+C in DNA, and base sequence of 16S ribosomal RNA gene (16S rDNA).
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  • Tomoko Aizawa, Noriyuki Iwabuchi, Aki Kikuchi, Makoto Urai, Hirosi Anz ...
    2001 Volume 15 Issue 1 Pages 6-10
    Published: July 25, 2001
    Released: March 01, 2008
    JOURNALS FREE ACCESS
    A rapid and simple method using the detergent Triton X114 to prepare outer membrane proteins from whole cells of bacteria containing mycolic acids, α-alkyl, β-hydroxyl fatty acids is described. SDS-PAGE analysis and subsequent N-terminal amino acid sequencing revealed that the proteins prepared from whole cells by this method were almost identical to outer membrane proteins prepared by the conventional cell-disruption and differential-centrifugation method. Therefore, this method is useful to prepare outer membrane proteins from whole cells of Mycolata, bacteria containing mycolic acids.
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