This paper reports taxonomical studies on a soil isolate, the strain MK703-102F1, which produces a new antimycobacterial antibiotic tubelactomicin. We propose that this strain should belong to a new species of the genus Nocardia with the new name Nocardia vinacea sp. nov., the type strain being MK703-102F1 (= JCM 10988 = IFO 16497). This new species was characterized by meso-diaminopimelic acid as a cell wall diamino acid, arabinose and galactose as whole-cell sugars, N-glycolated muramic acids in cell walls, type PII phospholipids, MK-8(H4) menaquinone, fatty acid components consisting of hexadecanoic (16:0), octadecenoic (18:1), 10-methyloctadecanoic (10Me-18:0) and hexadecenoic (16:1) acids, mycolic acids with 48–56 carbons, some physiological characteristics, 65 mol% G+C in DNA, and base sequence of 16S ribosomal RNA gene (16S rDNA).
A rapid and simple method using the detergent Triton X114 to prepare outer membrane proteins from whole cells of bacteria containing mycolic acids, α-alkyl, β-hydroxyl fatty acids is described. SDS-PAGE analysis and subsequent N-terminal amino acid sequencing revealed that the proteins prepared from whole cells by this method were almost identical to outer membrane proteins prepared by the conventional cell-disruption and differential-centrifugation method. Therefore, this method is useful to prepare outer membrane proteins from whole cells of Mycolata, bacteria containing mycolic acids.