Online ISSN : 1881-6371
Print ISSN : 0914-5818
ISSN-L : 0914-5818
Volume 23 , Issue 1
Showing 1-3 articles out of 3 articles from the selected issue
Original Articles
  • Nareeluk Nakaew, Wasu Pathom-aree, Saisamorn Lumyong
    2009 Volume 23 Issue 1 Pages 1-7
    Published: June 25, 2009
    Released: June 25, 2009
    [Advance publication] Released: May 15, 2009
    An actinomycete, designated as CMU-PNK470, was isolated from the soil in Phanangkoi cave in northern Thailand and subjected to phenotypic and genotypic characterization. Morphological and chemical properties indicated that this isolate belonged to the genus Spirillospora. Phylogenetic analysis based on 16S rRNA gene sequencing confirmed its placement in the genus Spirillospora and it was most closely related to Spirillospora albida (98.86%). This is the first reported isolation of Spirillospora albida from a cave habitat. The crude extract of this strain showed antimicrobial activity against three Gram-positive bacteria, Bacillus cereus and Methicillin-resistant Staphylococcus aureus with a MIC value of 23.1 μg/ml and Paenibacillus larvae with a MIC value of 185 μg/ml. It also reduced the number of viable Human Small lung cancer cells (NCI-H187) to less than 50% at a concentration of 10.18 μg/ml.
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  • Kimika Iwai, Susumu Iwamoto, Kazuo Aisaka, Makoto Suzuki
    2009 Volume 23 Issue 1 Pages 8-15
    Published: June 25, 2009
    Released: June 25, 2009
    [Advance publication] Released: May 29, 2009
    To collect new kinds of microorganisms for screening of biologically active substances, we focused on spider materials (webs, cuticle, egg sac), previously uninvestigated sources of such organisms. Using a new method of pre-treatment with 70% ethanol, 1,159 strains of actinomycetes were isolated from 196 spider materials, based on their morphological features. Of these, 293 strains were identified as non-filamentous actinomycetes from their 16S rRNA gene sequences. More detailed examination indicated that 139 strains belonged to the suborders Micrococcineae, Frankineae and Propionibacterineae, and they included some novel strains of non-filamentous actinomycetes. Thus, spider materials provide a more useful source of non-filamentous actinomycetes than do soil samples.
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