In order to clarify the role of amoebocytes in the midgut of the hard clam, Meretrix petechialis, the behavior of these cells and changes of pH value were investigated after feeding granules of different sizes such as yeast, potato starch, rice powder, kaolin or diatom earth. After being immersed in seawater suspended with those materials for 24 hours, the midgut of the hard clam was cut open. The total number of amoebocytes in the midgut were counted with THOMA's hemocytometer after staining with LOFFLER's methylene blue. The pH value was determined with the test paper (BTB or MR). Changes in total number (AT) and relative frequency (R) of amoebocytes, which was indicated by the ratio of AT in fed group to AT in control group, as well as pH value in each fed group are summarized as follows. AT or R varied with the size of the materials supplied, i.e., R was 80-160 when the particle size was 3-70 μm, and R ascended over 200 when the size exceeded 130 μm. No difference in AT or R was found between the groups supplied with organic materials and those supplied with inorganic ones. There was no relationship between AT and pH value in any group.
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