Cholic acid, taurine and cholesterol were identified from the bile of
Seriola quinqueradiata belonging to Toleostei. Another sterol series crystals of m.p. 164.5° were obtained which corresponded approximately to formula C
24H
42O
5, [α]
D20=+77.01° and were not precipitated by digitonin.
Crude cholic acid separated from the bile of
Eumetopia tubata belonging to marine mammals, was purified as a Ba-salt and a free acid, m.p. 193°, was isolated from the soluble Ba-salt. This acid corresponds to formula C
27H
46O
6, has [α]
D20=+41.89° and gives positive reactions to Milius, Hammasten and Libermann tests. Acetylation gives tetraacetate and oxidation by CrO
3 in glacial acetic acid gives triketo-monocarboxylic acid which corresponds to 3, 7, 12-triketocholanic acid, an oxidation product of cholic acid. It is assumed that the side chain was cleaved at C
24-position so that the authors assumed following structure for the substance with m.p. 193°.
抄録全体を表示