For the purpose of elucidating the mechanism of biochemical genetic of the yellow pigments losing mutation of
Pen. chrysogenum Q 176 to Pigmentless sultant
Pen. chrysogenum Q 176 ARIMA et OGASAWARA, cultural conditions of the yellow pigment production of
Pen. chrysogenum Q 176 by extinction of culture media-by Pulfrich photometer, and paper-chro-matography of the pigments were examined. Effects of carbohydrate sources, nitrongen sources, C:N ratio, rate of aeration, initial pH of culture media, each representative media for penicillin production, cultural temperature, inorganic salts on the production of pigments, and inhibitive action of NaCl, relation between pH and extinction of the pigments were examined.
From the above results, the pigment production and penicillin production seemed to be parallel, but unlike in the case of penicillin, pigments does not run down rapidly after reaching its peak, but keeps its level.
By selection of cultural conditions, it was possible to increase pigment production 2 or 3 folds of the control, but not so much as 10 folds or more as in the case of penicillin production.
Next, paper chromatographic study of the yellow pigments, especially the experimental conditions were examined. Final conditions were as follows: Toyo filter paper No. 50, size 2×40cm, pretreated with SÖRENSEN phosphate buffer solution at pH. 7. 7, developing solvent (butanol 90cc, isopropanol 10cc, water 18cc (80% saturated), developing hour 17_??_18 hrs., room temperature.
The fractionated spots on the paper stripe can be detected by the naked eye, FeCl
3 reaction or ultraviolet absorption spectrum of the extract from the spot by Beckman's quartz spectrophotmeter.
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