With a view to make early laboratory diagnosis of the influenza, HA production test of influenza virus and isolation of virus from patient's samples were carried out by use of the MDCK cell suspension, with the following results obtained.
The best HA production was obtained for both influenza virus A and B in 2×10
5 cells/ml of cell suspension. Fluid maintenance medium was Eagle's MEM cotaining twice as much glucose, four times as much vitamins, 20μg/ml of trypsin and 10% tryptose phosphate broth. Thus, using this method, virus isolation was made from the samples of some patients during influenza epidemics, and as a result, 16 strains of virus were isolated from 70 samples of first passage with results favorably comparable with such a method as using MDCK cell monolayers or fertile hen's eggs.
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