1) Five kinds of basic antagonistic substances, which resembled streptothricin in nature, were obtained in a form of hydrochloride, crystal-line helianthate and reineckate, and the identification between these sub-stances was performed.
2) All of these substances had rather lower potencies than strepto-mycin, in a dilution titer against
E. coli, while the substances No. 259 and No. 120 (Seki) were higher inhibitory against
Staphylococcus (Terashima strain) than streptomycin.
3) Six substances, including streptomycin, were arranged in the order of increasing toxicities as follows:
-36, streptomycin, 120 (Seki), 24, 39, and 259. The substance No. 36 was able to be injected both, intravenously and intramuscularly into mice in an amount of 20mg without showing any toxic signs. In this respect, the substance No. 36 has advantages over streptomycin.
4) The decomposition points of helianthates or reineckates of 4 streptothricin-Tike substances were found to be in the vicinities of that of streptothricin except No. 36 (Roseomycin). In reference to the other biological and chemical properties, however, the 5 substances seem quite different from streptomycin and from each other.
5) Maltol and Sakaguchi reactions were found to be positive in streptomycin alone, while glucosamine and other sugar reactions were positive in both streptomycin and streptothricin-like 5 substances. The chemical reactions positive in streptothricin group only, were not found yet.
6) A strain of
E. coli, which was made resistant to the hydrochlorides of 4 substances (36, 20, 259, and 120) respectively was resistant to each of 5 substances and somewhat less resistant to streptomycin too, while the same strain of
E. coli, which was made resistant to streptomycin, was not resistant to each of 5 substances. In this respect it seems very probable, that each of 5 substances resembles closely in their mechanisms of the anti-biotic functions.
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