In this study, a convenient and stable measurement method of cell concentration of Amphidinium sp. growing in culture solution was developed and it was applied to investigation of effects of different light property on growth rates of the algae. As cell clusters of the algae were observed when the concentration was high, the cell concentration measured with a conventional hemocytometry was underestimated. However, high linear relationships were observed between cell concentration and chlorophyll fluorescence intensity excited at 470 nm light and measured at 680 nm in spite of high concentrations. The relationships were represented as the two regression lines. The one belonged to the algae in the white, blue and red-blue light LED conditions and the other belonged to it in the red light LED conditions. The growth rate of the algae cultured under blue light was much larger than that under red light, and that under red-blue light (red:blue = 4:1) was larger than that under blue light. These results imply that blue lights are necessary for the algae to proliferate rapidly, and even a bit of blue light has the effect of propagate the algae, and chlorophyll fluorescence measurement could be applied to estimation of normally-developed algae cell concentration.

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要旨:肝細胞癌診断における白血球粘着阻止試験(LAIテスト)の反応機構の解析を行った.まず,LAI陽性肝細胞癌症例の白血球をリンパ球分画と好中球分画に分離して行うplate法による2段階LAIテストを施行したところ,hepatoma抗原に反応してリンパ球分画よりchemical mediatorが遊出され粘着阻止に働くことが判明した.次に,このLAI現象をblockする血清中のblocking factorをSephadex G 200 column chromatographyにより分析したところvoid volumeに存在していることが判明し,antigen-antibody complexの状態になっていると考えられた.次にMeyer zum Buschenfeldeのliver specific antigenの分析手段を応用し,LAIテストに使用したhepatoma extractの抗原性について検討した.その結果,LAI活性はSephadex G 100のfirst peakに存在し,さらにその分画をSephadex G 200にて分析したところ,その活性はfirst peakに存在していた.この結果から,LAI活性を示す分画は肝癌細胞の膜成分に一致する分画であるとの結論を得た.

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