In the field of light microscopy, there have been some reports on the rat kidney with riboflavin-deficiency. That is the swelling and vacuolation of the proximal tubular epitherium. But no reports have been found in the field of electronmicroscopy.
For the purpose of getting precise information on ultrastructural changes in the proximal tubular epitherium associated with riboflavin-deficiency, histopathological study was performed on the first hand and next its results and findings was reexamined by electronmicroscopy in the most finest point of view.
The deficient state of riboflavin was induced by riboflavin deficient diet and by biological antagonist against riboflavin (6, 7-dimethyl-9-(2 '-hydroxyethyl)-isoalloxazine) in rats.
The results obtained are as follows.
1) Morphological changes in riboflavin-deficient kidney induced by the diet as well as the antagonist were almost same.
2) Predominant changes in proximal tubular cells were observed by the light microscopy. Tubular lamina was enclosed by the marked hydropic swelling of the tubular cytoplasma. Other part of tubules, glomerulus and stroma were almost similar in appearance to those found in control rats.
3) In the field of electronmicroscopy, the epitherium of proximal tubular cells also showed marked changes. The cytoplasmic matrix were extremely decreased in its electron-density. The mitochondria were decreased in number and size, and had lost their original uniformity, so asymmetric irregular shaped ones were observed. The mitochondrial cristae appeared to have lost their integrity and under-gone fusion.
Transport vesicles and vacuoles in cytoplasma, and infoldings of the basal plasma menbrane were prominently decreased in number, and were diminished or obliterated.
Most cytoplasmic bodies contain irregular fragments or strands of dense filamentous or menbranous materials.
The brush border, endoplasmic reticulum and Golgi apparatus were similar in appearance to those found in control rats.
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