1. Glycolytic intermediates and adenine nucleotides in polymorphonuclear granulocytes from guinea pig peritoneal exudate were analyzed. Glucose 6-phosphate, fructose 6-phosphate, fructose 1, 6-diphosphate, dihydroxyacetone phosphate, glyceraldehyde 3-phosphate, 3-phosphoglycerate, 2-phosphoglycerate, phosphoenolpyruvate, pyruvate, ATP, ADP and NAD
+ were measured enzymatically using a fluorometer. Nucleotides were also analyzed by column chromatography on Dowex 1-formate. Peritoneal exudate was deproteinized either immediately after removal from the body to obtain an aerobic steady state pattern or after incubating with cyanide to get an anaerobic steady state pattern.
2. Mass action ratios of glycolytic reactions in leucocytes were calculated from the above data and rate-limiting steps in the cellular glycolysis were discussed. Hexokinase [EC 2. 7. 1. 1], phosphofructokinase [EC 2. 7. 1. 11] and pyruvate kinase [EC 2. 7. 1.40] were found to be major rate-limiting steps in leucocytes as in the case of erythrocytes.
3. Following changes were observed when leucocytes were incubated in presence of cyanide: 1) decrease in hexose monophosphates, 2) increase in fructose diphosphate and triose phosphates, 3) decrease in monophosphoglycerates and phosphoenolpyruvate and 4) slight decrease in ATP and increase of ADP.
抄録全体を表示