This study aimed to determine the prevalence of fecal carriage of extended spectrum β-lactamase (ESBL) and/or plasmidic AmpC β-lactamase (pAmpC) producing Escherichia coli among dogs (n=428) in Turkey. Polymerase chain reaction (PCR) and sequencing were used to characterize genes encoding β-lactamase and plasmid mediated quinolone resistance (PMQR). Antimicrobial susceptibility testing and PCRs for virulence genes and phylogenetic groups were also performed. Cefotaxime resistant

. coli isolates were detected in 95 (.2%) of the swab samples. Sequencing analysis results showed occurrence of various β-lactamase genes: bla_CTX-M-15 (62), blaTEM-

1b

(42), bla_CMY-2 (

22

), bla_CTX-M-3 (16), bla_CTX-M-1 (15), bla_OXA-1 (

9

) and bla_SHV-12 (3) alone or in combination. The most frequently encountered phylogenetic group was group A₁ (35.

8

%), followed by group D₂ (

22

.1%),

B

1 (15.

8

%), D₁ (

9

.

5

%),

A₀

(

7

.4%),

B2₂

(

5

.3%) and

B2₃

(4.2%), respectively. PMQR genes, aac(6’)-Ib-cr, qnrS1 and qnrB10 were detected in 25.3, 10.

5

and 1.1% of the isolates, respectively. While all isolates were susceptible to imipenem and amikacin, resistance rates to non-β-lactam antibiotics ranged from 20.

0

% for tobramycin to 56.

8

% for tetracycline. The virulence genes were only detected in 34 (36.2%) of the isolates and this isolates carried single or various combination of virulence genes of iucD, papC, papE,

f17a

-A and eaeA. Four isolates were identified as human virulent pandemic CTX-M-15 producing

E

. coli clone O25

b

:ST131/

B

2. To the best of our knowledge, this is the first study to show fecal carriage of ESBL/pAmpC type β-lactamase producing

E

. coli isolates among dogs in Turkey.

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Six new cembranoids sarcophytol P (3), R (4), S (), K (), (11), and T (13) were isolated from the soft coral Sarcophyton glaucum. Sarcophytol P (3) was shown to be the 20-hydroxy derivative of the major component sarcophytol A (1), and afforded the cyclization product 6 in CHCl_3 at room temperature, in a same way as in 1. Sarcophytols R (4) and S () were correlated to 1, by conversion of its R,R and S,S epoxide derivatives. Sarcophytol K() was a 13, 14-dihydroxycembranoid having a 1,3Z-diene moiety. The absolute configuration of and its 1Z,3- and 1Z,3Z-isomers sarcophytol (2) and J (10) were determined by circular dichroism study of their bis-p-dimethylaminobenzoate derivatives. Sarcophytols (11) and T (13) were 1- and 1,3Z-isomers of 1. Compound 11 showed characteristic broadening of the ^1H-NMR chemical shifts, due to the restricted conformational interconversion. Using the three cembranoids sarcophytols (11, 1,3), N (15, 1Z,3Z) and T (13,1,3Z), spontaneous autoxidation-cyclization, in CHCl_3,was examined, in order to compare the stereochemical course of the reaction with that of 1 (1Z,3), which affords trans-fused bicyclo[.3.]tetradecene systems. The 1,3Z-isomer 13 gave the same cyclization product 18, as in the reaction of 1, even though it is isomeric at C-1,3. The 1Z,3Z isomer 15 gave 19, in consequence of the reversed geometry at C-3 of 15. The 1,3-isomer (11) gave the bicyclic product , having an antipodal fusion as compared with 19. This was confirmed by PCC oxidation of 19 and , giving enantiomeric ketones 23 and 24 respectively. The 1(14)-epoxide 26 was shown to be the immediate precursor of , and acounted for the inversion of the geometry at C-1 of the cyclization product. Compound 26 is isomeric with the epoxide 17 derived from 1. The epoxide 17 is the postulated precursor in the conversion of 13 to 18. It is noteworthy that the mode of antipodal fusion of the cyclopentane rings, in 18 and , was controlled by the chirality of the epoxy rings. The C-14 hydroxyl group participates in the transannular cyclization, but was found not to be the requisite functional group for the reaction. Similar treatment of cembrene C (30), the parent hydrocarbon of 1, also reacted in CHCl_3 giving the bicyclic product 32.

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A long term follow-up of patients after antero-lateral partial vertebrectomies (ALPV) was performed using patient charts and questionnaires. During the past 15 years, ALPV has been performed on 159 patients with cervical spinal disorders. Among them, 136 patients whose survey was performed more than 1 year ago were selected. were male and 44 were female. Their ages ranged from 41 to 82, and the average age was 63.1. The follow-up period was from 1 to 14 years (the average being 3. years). Pathology included spondylosis 119, OPLL 31, canal stenosis and combined lesion cases. Surgical resuts were evaluated using a grade scale (excellent ; , good ; G, fair ; , poor ; P and dead ; D). At discharge, group was 40.2%, G was 51.%, was .2%, P was %, D was .%. At the end of the follow-up period was 36.%, G was 35.%, was 20.2%, P was .1% and dead was .%. None of the deaths were related to the surgery. However, the score obtained from the Neurosurgical Cervical Spinal Scale were essentially consistent. Results of the questionnaire showed that 61.4% of the patients were satisfied, 30.1% were fairly satisfied and only .4% were dissatisfied. Dissatisfied cases included 2 cases of surgical failure but most of them were related to the progressive worsening of other lesions such as thoracic, lumbar spinal lesions or peripheral neuropathy. In 2 cases reoperation was performed, one anterior fusion with fibular strut graft for kyphoscoliosis and the other anterior fusion with a mini-plate for a fracture of the lamina and slippage of the vertebral body. In some cases, weakness of the C root area was encountered but most of these were resolved in a few months. Radiologically, the bony defects of the vertebral body were observed to become smaller, namely they were replaced by new growth of bone slowly in all cases. Thus, ALPV seems to be a useful surgical procedure for multilevel cervical spinal disorders.

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The ability of Climacodon septentrionalis to immobilize and kill a mycophagous nematode (Aphelenchoides sp.) in vitro is described for the first time. Two isolates produced droplets (20–45 μm in diameter) that formed at the apices of tall, stalked, and branching secretory cells (700–1,500 μm tall). On 2% modified malt extract agar, nematodes became enveloped in the droplets, which restricted their ability to move and resulted in complete immobilization and death within several hours of contact. The rate of decomposition of the nematodes varied considerably, with most individuals persisting for weeks whereas others were degraded within several days and appeared to be colonized by dense hyphal growth. This study provides the first documentation of a non-agaricoid fungus producing secretory cells that are able to immobilize nematodes.

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This study has been carried out to decide the reinforcement method of the impacted concrete pile by the pile driving.
From the analysis of measured values of the dynamic strain on the pile head, the following conclusions have been obtained.
Moreover, the author wishes to promote this study and to find out the behaviours of the pile driven in the ground.
(1) The relation between the dynamic energy of the hammer and the dynamic strain in the pile.
ε_V.100=(1-k)/EA(S+R/2) (1)
ε_V.50=(1-k)/EA(S+R) (2)
where ε_V.100=the dynamic strain at the point of 100cm from the top of the pile
ε_V.50=dynamic strain at the point of 50cm from the top of the pile
=dynamic energy of hammer
A=sectional area of the pile
=Young's modulus of concrete
S=penetration
R=rebound
(1-k)=efficiency of the dynamic energy of hammer
W=hammer weight
H=hammer stroke
For , (1-k) and in Eqs. (1) and (2), the following values in Table I and Table II is available.
Table I
Table II
F_c=ultimate compressive strength of concrete
(2) The relation between the sectional area of the pile and the dynamic strain of the head of the pile.
When the PC pipe pile (350mmφ or 300mmφ) is driven in the ground with the Type diesel hammer, the value of ε_V.50 is assumed by Eq. (3)
ε_V.50=(987-.57A)×10^-6 (3)
where, A=equivalent sectional area of the pile (cm²)
(3) The distribution of the dynamic strain of pile head
The axial dynamic strain ε_V.x at the point of distance x from the top of pile is expressed as the following general Eq. (4).
ε_V.x=ax-

b

(4)
where, a and

b

=constant characteristic
Then the dynamic strain of PC pile, which is driven with diesel hammer

22

, is expressed in Eqs. (

5

) or (6).
For PC pile (D=300mmφ, t=70mm)
ε_V.x=

9

.02×10⁴×x-

0.0897

(

5

)
For PC pile (D=350mmφ, t=80mm)
ε_V.x=10.

92

×10⁴×x-

0.152

(6)
(4) The relationship between the vertical (axial) dynamic strain ε_V and horizontal dynamic strain ε_H.
The value of ε_H/ε_V at the point (such as x=10cm), near the top of pile, shows the variable value as ε_H/ε_V=

0

.1∼

0

.4.
But ε_H/ε_V values at 35cm point and at 70cm point from the top of pile become the constant values as follows:
For RC pile (ε_H/ε_V)=

0

.2 (

7

)
For PC pile (ε_H/ε_V)=

0

.24 (

8

)
(

5

) The suggestion for the reinfocement range of the head of pile
By assuming the ultimate compressive strain of concrete as ε_cu=2500×10^-6 and the ultimate tensile strain as ε_tu=125×10^-6 and applying ε_tu and ε_cu to the Fqs. (

5

), (6), (

7

) and (

8

), the author has suggested the following idea.
1) The minimum width of the iron band to reinforce the pile head is necessary to be in the range of 20∼30cm.
2) The pile must be specially reinforced by spiral hoop at least 100cm in length from the top of the pile head.

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Reactions of nor-diterpenoid dilactones isolated from Podocarpus plants have been investigated. The : ,: 11-dienolide type [C] dilactones, the least available but biologically the most active members, are mainly derived from naturally abundant dilactone components of α-pyrone type [A] and : -epoxy-: 11-enolide type [] for total-synthetic and biological purposes. From nagilactone (), for example, a combined sequence of hydrogenolysis of : -epoxide group and dehydration gives nagilactone (), 3β-hydroxynagilactone () and 2: 3-dehydronagilactone (19), which have been found from natural sources. From nagilactone A (1), photo-hydration and concomitant double bond migration of its acetate results in a lactol (), which is reduced to 1β-hydroxynagilactone (), another natural type [C] dilactone. Derivation of the type [A] or [] from others is not successful. Some attempts for conversion of the ring A functional groups have also been studied.

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The phase diagrams of the Ni-Mo- and Ni-W- ternary systems in the region of less than 50mol% were constructed by thermodynamic calculation, based on the data obtained by thermodynamic measurement of the related materials. We found three ternary eutectic points and three or two ternary peritecto-eutectic points as follows:
:L (1365K, 71.mol%Ni-6.mol%Mo-.mol%)=(Ni)++
:L (1355K, 62.mol%Ni-2.mol%Mo-30.mol%)=++
:L (1445K, 42.mol%Ni-30.6mol%Mo-10.3mol%)=(Ni)+NiMo+
P₁:L (1812K, 34.mol%Ni-42.3mol%Mo-.mol%)+MoB=+
P₂:L (1633K, 42.3mol%Ni-40.4mol%Mo-17.3mol%)+Mo=+
P₃:L (1812K, 53.mol%Ni-33.mol%Mo-12.mol%)+Mo=NiMo+
:L (1622K, 51.mol%Ni-31.6mol%W-17.4mol%)=(Ni)+W+
:L (1260K, 71.mol%Ni-.mol%W-.mol%)=(Ni)++
:L (1291K, 65.4mol%Ni-4.mol%W-29.mol%)=++
P₁:L (2115K, 23.mol%Ni-43.1mol%W-33.1mol%)+WB=+
P₂:L (1657K, 48.mol%Ni-33.1mol%W-18.mol%)+=W+
The calculated phase diagrams are expected to be useful for the development of new Ni-based heat-, corrosion- or wear-resistance alloys.

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Four new sterols, , 6α-epoxy-(, 24R)-ergosta-(14), -diene-3β, -diol (1), (, 24R)-ergosta-, -diene-3β, , 6β, -tetrol (2), (, 24R)-ergosta-, (11), -triene-3β, , 6β-triol (3) and 3β, , 6β-trihydroxy-(, 24R)-ergost--en--one (4), have been isolated from the fruit bodies of Grifola frondosa (FR.) S. . GRAY (Polyporaceae)together with fourteen known ones (-18), of which two ( and 6) are reported for the first time from a natural source. The structures of these compounds were elucidated on the basis of spectral data.

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We compared three groups of pregnant women: placebo with normotensive women, group A which included preeclamptics, and group which comprised preeclamptics who were supplemented their diets with vitamins C and . MDA increased from 6. ± 2. (placebo) to .48 ± 1.2 (A) and .02 ± 1. nmol/gHb (). NO concentrations were enhanced from 19.3 ± 4.2 (P) to 23. ± 6.4 (A) and 24.1 ± .4 μmol/L (). GSH contents were decreased from 10.42 ± 2.81 (P) to .02 ± 2. (A) and .39 ± 1.02 μmol/g Hb (), whereas GSSG concentrations increased from .98 ± .28 (P) to 1.24 ± .29 (A) and 1.08 ± .12 μmol/g Hb (). SOD activity decreased 23% in A and 14% in ; GRx decreased 27% in A and .% in ; GPx decreased 12% in A and .6% in . Catalase activity, however, increased 27% in A and 29% in as compared to control. Thus, we conclude that the use of vitamins C and should be considered for the control of certain important biochemical indices during the development of preeclampsia; however, further studies are needed to develop methods for the prevention of preeclampsia in women at high risk.

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The gamma-ray spectrum and the conversion-electron spectrum are measured in the decay of the Tb¹⁵² activity. The L-subshell electron lines of the 586. keV +→2₁⁺ transition are separated by the ion-free beta-ray spectrometer. The M1-2 mixing ratio, δ², and the dimensionless ratio X of -2 mixing of the 586. keV transition are obtained to be δ²≤.16 and .034≤X≤.038, respectively. The 2 branching ratio (2; +→+): (2; +→2₁⁺): (2; +→0₁⁺) is determined to be .023 : 1. : .6.

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Three new azaphilone derivatives designated falconensins (), (6), and G () were isolated from the mycelium o Emericella falconensis, along with falconensins A (1), (2), C (3), D (4), and H (), and three hopane-type triterpenes, zeorin (), hepane-, -diol (10), and hopane-6α, , -triol (11). The structures of - were confirmed by spectroscopic investigation and chemical correlations. The absolute stereochemistry of falconensins A (1) to G() was also established.

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Vibrio parahaemolyticus is a prevalent food-borne pathogen in Taiwan, Japan and other Asian countries. This work presents a novel ribotyping method for the molecular epidemiological examination of this pathogen. Genomic DNA was fragmented by HindIII digestion and hybridized with cDNA probe for Escherichia coli 16S and 23S RNA genes. A total of 121 isolates obtained from outbreaks during 1992 and 1994 in Taiwan were characterized by this ribotyping method. Four to seventeen restricted fragments were visualized in these isolates. After hierarchical cluster analysis, these isolates were grouped into thirty different ribotypes. In addition, A3, A, 3 and 1 were the major ribotypes, consisting of .3, 13.2, .1, and .3% of the isolates, respectively. A, , , G and were the major groups, consisting of 46.2, 14., .1, 6., and 6.% of the isolates, respectively. The discriminatory ability of this ribotyping method, as determined by Simpson's index of diversity, was .93, which closely resembled that of a previously reported pulsed-field gel electrophoresis method.

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A full account is given of the chemical behavior observed for , -dialkyladeninium salts (16). On treatment with boiling 1N aqueous NaOH for 60min, 16a, , d, (X=I), 16c (X=Br), and 16 (X=ClO₄) rearranged to isomeric N⁶, -dialkyladenines (21a-) in 50-91% yields. Treatment of the salts with .N aqueous Na₂CO₃ at room temperature for 30-90min or with Amberlite CG-400 (OH^-) in H₂O at room temperature gave the ring-opened derivatives a- (in the trans-formamide form) in 56-83% yields, and rate constants for the ring-opening reactions of 16a, , d-g (X=ClO₄) and 16c (X=Br) leading to a-g were determined in H₂O at pH .84 and ionic strength .50 at 25°C. Cyclization of a with NaH in AcNMe₂ at room temperature or with boiling 1N aqueous NaOH produced 21a in 84% or 72% yield, respectively.In solution, the trans-formamides seemed to transform slowly into the cis-formamides 23, attaining equilibria. The existence of such an equilibrium in D₂O or Me₂SO-d₆ at 25°C or in H₂O at pH .84 and ionic strength .50 at 25°C was kinetically confirmed in the case of a, and the mechanism of the rearrangement of 16 to 21 through is discussed on the basis of the above kinetic results and Deslongchamps' theory of stereoelectronic control. On treatment with NaBH₄ in MeOH at room temperature, 16a (X=I) furnished the , -dihydro derivative 28 (84% yield), which slowly decomposed in H₂O at 60°C to give a in 49% yield.The , -dialkyladeninium salts (16) were found to be obtainable from N'-alkoxy-1-alkyl--formamidoimidazole-4-carboxamidines () through an alternative synthetic route : Alkylations of with alkyl halides in HCONMe₂ in the absence of base, followed by hydrogenolysis of the N'-alkoxy group and cyclizatio (or vice versa) produced 16 in acceptable yields. In order to interpret the proton nuclear magnetic resonance spectrum of a, the 2-deuterated species 26 was also synthesized from 24 via 25 and 27.

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The gamma-ray spectrum and the conversion-electron spectrum are measured in the decay of the ^116mIn activity. Directional correlations are also measured for 818–1293 and 1097–1293 keV cascades. The M1-2 mixing ratio δ, and the -2 mixing ratio μ_k are obtained for 818. keV +→2₁⁺ transition to be δ=1.52−

0.22

+

0.26

, and μ_k≤6.1×10⁻⁴ respectively. The

E

2 branching ratio

B

(

E

2;

2₂

+→

0_g

+):

B

(

E

2;

2₂

+→2₁⁺):

B

(

E

2;

2₂

+→0₁⁺) is determined to be

0

.0158:1.

0

:

5

.86.

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Melithasterols A-D (1-4), isolated from a gorgoinan coral Melithaea ocracea of Okinawa, were shown to be cholestane-, 24-methyl--dehydrocholestane-, -dehydrocholestane-, and 24-methylenecholestane-derivatives. respectively, having a common 3β, α -dihydroxy-α, 6α-epoxy-Δ^ steroid nucleus. This was confirmed by direct comparison with the authentic sample prepared by lead tetra-acetate oxydation of cholest-6-ene- 3β, α, α-triol 3-monoacetate. Twelve new polyhydroxysterols were isolated from an Alcyonium sp. (,,), and Sclerophytum sp. (14,16,19,20,21,a,23,26,28) soft corals collected off the coasts of the Andaman and Nicobar Islands in the Indian Ocean. All the Sclerophytum sp. soft corals contained 17a or its 25-deacetyl derivative. Compounds to were identified as 24-methylenecholest--ene- 3β, 16β-diol-3--α-L fucoside () and its β-() and α-hydroxy () derivatives. Compound 14 was shown to be 24S-24-methylcholest--ene-3β,25ξ,26-triol and was correlated to the known compound 15 by α, 6β-glycolation. Compound 16 was shown to be β, 6α-isomer of 17, correlating to 17 by PCC oxidation followed by dehydration. Compound 19 was identified with 25-deacetyl derivative of the known compound lobosterol (19a) by hydrolysis of authentic lobosterol. Compounds 20 and 21 were shown to be -dehydro-(20) and -dehydro-(21) derivative of 17a and 17, respectively, by comparisons of their spectral data with reference compounds. Compounds a and 23 were 24S-methylcholestane-3β, α 25-triol-6-one 25-monoacetate (a) and its 25-deacetoxyl derivative (23). Partial PCC oxidation of 17a afforded a. Compounds 26 (andamansterol) and 28 (nicobarsterol) were shown to be gorgost--ene-3β, α. 11α, 21-tetrol and novel secosteroid, 11,21-cyclo--homo-11-oxa-, 11-secoergostane-3β, 6α, 12ξ-triol--one, respectively, by spectral analyses (H-HCOSY, HMQC, HMBC).

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2000年月から月にかけて, 4県 (静岡, 埼玉, 秋田および新潟) で採集した市販鶏肉 (むね肉, もも肉および手羽先) 計128検体におけるカンピロバクター (Campylobacter jejnni/coli) の汚染率は75.%(96/128) であり, 汚染菌数 (MPN法) は陽性96検体中検体 (95.%) において10³cfu/100g以下であった.また, 分離されたカンピロバクター-192株はC.jejuni (182株, 94.%) およびC.coli (10株.2%) に同定され, Penner法による血清型刷では, 群株 (11.%), 群16株 (.3%), C群15株 (.%) などで, 型別不能株は47株 (24.%) であった.

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