2010 Volume 16 Issue 5 Pages 409-416
Endogenous tocopherols in extracted lipids from four cultivars of broad beans (Vicia faba) were determined by high-performance liquid chromatography. The molecular species and fatty acid (FA) distribution of triacylglycerols (TAG) isolated from their total lipids were analyzed by a combination of AgNO3 thin-layer chromatography (AgNO3-TLC) and gas chromatography (GC), and then examined in relation to the tocopherol distribution. γ-Tocopherol was present in the highest concentration (51.2-55.6 mg/kg beans), followed by α-tocopherol (15.2-16.3 mg/kg beans) and δ-tocopherol in small amounts (1.2-1.5 mg/kg beans). Fourteen TAG molecular species were identified and quantified by successive applications of AgNO3-TLC and GC. Oleodilinolein (18.9-21.8%) and trilinolein (21.0-23.9%) were the main components; the other species were found in minor quantities (less than ca. 10.0%). These results suggest that the tocopherol content, FA distribution and TAG molecular species in broad beans are not dependent on the cultivation areas during the growing season.
