Anti-HSV Activity of Kuwanon X from Mulberry Leaves with Genes Expression Inhibitory and HSV-1 Induced NF-κB Deactivated Properties

Fang Ma; Wenwei Shen; Xiaoqi Zhang; Manmei Li; Yifei Wang; Yuxiao Zou; Yaolan Li; Hui Wang

doi:10.1248/bpb.b16-00401

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Anti-HSV Activity of Kuwanon X from Mulberry Leaves with Genes Expression Inhibitory and HSV-1 Induced NF-κB Deactivated Properties

Fang Ma, Wenwei Shen, Xiaoqi Zhang, Manmei Li, Yifei Wang, Yuxiao Zou, Yaolan Li, Hui Wang

Author information

Fang Ma
Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University
Wenwei Shen
Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University
Xiaoqi Zhang
Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University
Manmei Li
Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University
Yifei Wang
Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University
Yuxiao Zou
Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences
Yaolan Li
Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University
Hui Wang Corresponding author
Department of Microbiology and Immunology, Medical College, Jinan University

Keywords: anti-herpes simplex virus (HSV) activity, Kuwanon X, penetration, gene expression, nuclear factor (NF)-κB, Mulberry

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2016 Volume 39 Issue 10 Pages 1667-1674

DOI https://doi.org/10.1248/bpb.b16-00401

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Abstract

Six stilbene derivatives isolated from Mulberry leaves including Kuwanon X, Mulberrofuran C, Mulberrofuran G, Moracin C, Moracin M 3′-O-b-glucopyranoside and Moracin M were found to have antiviral effects against herpes simplex virus type 1 and 2 (HSV-1 and HSV-2) at different potencies except for Mulberrofuran G. Kuwanon X exhibited the greatest activity against HSV-1 15577 and clinical strains and HSV-2 strain 333 with IC₅₀ values of 2.2, 1.5 and 2.5 µg/mL, respectively. Further study revealed that Kuwanon X did not inactivate cell-free HSV-1 particles, but inhibited cellular adsorption and penetration of HSV-1 viral particles. Following viral penetration, Kuwanon X reduced the expression of HSV-1 IE and L genes, and decreased the synthesis of HSV-1 DNA. Furthermore, it was demonstrated that Kuwanon X inhibited the HSV-1-induced nuclear factor (NF)-κB activation through blocking the nuclear translocation and DNA binding of NF-κB. These results suggest that Kuwanon X exerts anti-HSV activity through multiple modes and could be a potential candidate for the therapy of HSV infection.

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