Enhancement of TFO Triplex Formation by Conjugation with Pyrene via Click Chemistry

Yosuke Taniguchi; Akira Tomizaki; Nozomu Matsueda; Hidenori Okamura; Shigeki Sasaki

doi:10.1248/cpb.c15-00570

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Enhancement of TFO Triplex Formation by Conjugation with Pyrene via Click Chemistry

Yosuke Taniguchi, Akira Tomizaki, Nozomu Matsueda, Hidenori Okamura, Shigeki Sasaki

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Keywords: triplex-forming oligonucleotide, artificial nucleoside, antigene

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2015 Volume 63 Issue 11 Pages 920-926

DOI https://doi.org/10.1248/cpb.c15-00570

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Abstract

This paper reports the preparation of 14-mer triplex-forming oligonucleotides (TFOs) containing a 2-O-methyl-1-β-phenyl-α-propargyl-ribose unit, which was conjugated with azide-modified molecules via a click reaction. Modification of these TFOs with pyrene assisted triplex formation, improving the stability of the triplex DNA and the anti-proliferative effects against A549 cells.

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