Comparison of the Growth Kinetics of Neuropathogenic and Nonneuropathogenic Equid Herpesvirus Type 1 (EHV-1) Strains in Cultured Murine Neuronal Cells and the Relevance of the D/N752 Coding Change in DNA Polymerase Gene (ORF30)

Souichi YAMADA; Tomio MATSUMURA; Koji TSUJIMURA; Tsuyoshi YAMAGUCHI; Kenji OHYA; Hideto FUKUSHI

doi:10.1292/jvms.70.505

Virology

Comparison of the Growth Kinetics of Neuropathogenic and Nonneuropathogenic Equid Herpesvirus Type 1 (EHV-1) Strains in Cultured Murine Neuronal Cells and the Relevance of the D/N₇₅₂ Coding Change in DNA Polymerase Gene (ORF30)

Souichi YAMADA, Tomio MATSUMURA, Koji TSUJIMURA, Tsuyoshi YAMAGUCHI, Kenji OHYA, Hideto FUKUSHI

Author information

Keywords: DNA polymerase, EHV-1, Neuron

JOURNAL FREE ACCESS

2008 Volume 70 Issue 5 Pages 505-511

DOI https://doi.org/10.1292/jvms.70.505

Details

Abstract

We compared the growth kinetics of neuropathogenic and nonneuropathogenic equine herpesvirus type 1 (EHV-1) strains in mouse cerebral cortex cells and investigated the relevance of the D/N amino acid change at position 752 of ORF30 in Japanese isolates. Neuropathogenic electropherotype P strains 01c1 and 89c25 exhibited similar growth kinetics to nonneuropathogenic P strain 90c16 in cultured neurons; however, the growth ability of type B strain 97c7 was lower than those of the other strains tested. The amino acid encoded at 752 of ORF30 in 01c1 was asparagic acid; asparagine was encoded in the other EHV-1 strains isolated from Japanese horses. The D/N₇₅₂ difference in ORF30 may not be related to replication ability in neurons.

Corresponding author

Register with J-STAGE for free!