抄録
Intracellularly synthesized polysaccharide from glucose-1-phosphate by phosphorylase and amylo-1, 4→1, 6-transglucosidase in rat liver cells was observed by histochemical and electron microscopical procedures.
This polysaccharide stained red-purple with iodine under light microscope. It was completely digested by α-amylase, while incompletely digested by β-amylase.
Electronmicroscopically, newly synthesized polysaccharide appeared to be finer granules than β-granules of native glycogen. These new granules were present as amorphous aggregates or monoparticulates, but very often had a tendency to make irregular rosettes, different from native glycogen. They stained less with lead in density on electron microphotograph and were more sensitive to electron beam than native glycogen particulates.
