1978 年 11 巻 4 号 p. 408-417
Epithelial cells from the weanling rat liver could proliferate easily if repeated colonial selection of the epithelial cells was performed in order to prevent overgrowth of the non-epithelial cells. The newly started two epithelial cell lines were studied ultrastructurally and cytochemically in order to examine whether or not these cells in vitro were hepatocytes. These cell lines possessed features common to other rat liver cell lines described in various studies. However, no definite evidence suggesting a hepatocytic origin was found, although some features were characteristic of the hepatocytes, especially those of the fetal rat. Cytochemically, these cells exhibited many types of enzyme activity, except for glucose 6-phosphatase. Cytochemical study of the cells in the primary culture suggested that the cell lines were not derived from the fully differentiated hepatocytes.
