SELECTIVE SEPARATION OF EPIDERMAL CELLS FOR CYTOFLUOROMETRIC ESTIMATION OF NUCLEAR DNA

抄録

A simple procedure used to isolate epidermal cells for the cytofluorometric estimation of their nuclear DNA content is described. The principal part of this method involves the microdissection of fixed tissue sections as well as ultrasonic irradiation. The loss of Feulgen-stainable nuclear DNA during treatment with ultrasonics, using lymphocytes and spermatozoa in control experiments, was estimated at approx. 2% of the original content. In using this method for determining the nuclear DNA content of human epidermis, it was found that the presence of individual cells was maximal in the stratum basale and minimal in the uppermost layer of stratum granulosum.