MONOAMINE-CONTAINING CELLS IN THE GASTRIC MUCOSA OF THE KITTEN: A NEW METHOD EMPLOYING COMPARATIVE FLUORESCENCE AND ELECTRON MICROSCOPY ON IDENTICAL CELLS
1979 年 12 巻 4 号 p. 383-390
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1979 年 12 巻 4 号 p. 383-390
Monoamine-containing cells and adrenergic nerve fibers are clearly demonstrated by fluorescence microscopy on semi-thin sections of Araldite-embedded materials previously fixed with a mixture of formaldehyde and glutaraldehyde. This enabled us to examine identical monoamine-containing structures by both fluorescence and electron microscopy.
Fluorescence microscopy reveals two kinds of monoamine-containing cells in the fundic mucosa of the kitten: one with yellow fluorescence and the other with blue-green fluorescence. Electron microscopically, yellow fluorescent cells are EC cells containing characteristic polymorphous secretory granules, while blue-green fluorescent cells have round secretory granules and are probably A, X and third type cells (Lausanne classification (9)).
