DIFFERENTIAL STAINING OF CADMIUM AND ZINC BY THIAZOLYLAZOPHENOLS
1979 年 12 巻 5 号 p. 460-464
詳細
1979 年 12 巻 5 号 p. 460-464
Thiazolylazocresol (TAC) was found to demonstrate cadmium and zinc in different colors in tissue sections, enabling differential staining by coloration without a masking agent. The metals in stained sections were microscopically identified by the aid of a laser microprobe.
The absorption spectra obtained from TAC-metal chelates in a test tube show three absorption maxima: around 650 nm (λ2), 550 nm (λ1) and 400 nm. The ratio of the absorption of λ1 and λ2 with cadmium differs considerably from that of zinc. The difference in the ratios (λ2/λ1) is presumed to be the cause of the differential staining by coloration.
