抄録
Neuronal structures containing glicentin- or pancreatic glucagon-like immunoreactivity were studied in the rat brain by peroxidase anti-peroxidase immunohistochemistry. With anti-glicentin 49-69 serum R-4804, which recognized gut type glucagon but did not react with pancreatic type glucagon, immunoreactive nerve fibers and terminals were distributed in several brain regions including the hypothalamus. No immunoreactive neuronal soma was found in intact rats. Intraventricular injection of colchicine successfully revealed cell bodies with glicentin-like immunoreactivity in the lower medulla oblongata. These neuronal somata, though few in number, were mainly localized in a region between the nucleus tractus solitarius and nucleus dorsalis nervi vagi. Ventrolaterally to this region, some positive cells were also scattered up to the nucleus reticularis lateralis. The location of these glicentin-like immunoreactive cells closely resembled that of catecholamine cell groups A1 and A2. Our results strongly suggest that the rat brain contains gut type but not pancreatic type glucagon, and here a map of glicentin-like immunoreactive neuronal structures was given in detail.
