ACTA HISTOCHEMICA ET CYTOCHEMICA
Online ISSN : 1347-5800
Print ISSN : 0044-5991
ISSN-L : 0044-5991
A STUDY OF POTASSIUM PERMANGANATE FIXATION FOR ELECTRON MICROSCOPIC DETECTION OF SMALL GRANULAR VESICLES IN THE SYMPATHETIC NERVOUS SYSTEM
JUNZO OCHI
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ジャーナル フリー

1969 年 2 巻 1 号 p. 13-18

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抄録
A 3% solution of potassium permanganate is effective at acid pH as a fixative for the small noradrenaline-containing granular vesicles of the symapthetic nervous system. The observed high electron density of the central granules in the vesicles is probably due to precipitated MnO2 which is reduced KMnO4 in a coupled oxidation of noradrenaline to noradrenochrome. A 3% solution of KMnO4 in dichromate buffer (pH 6.0) is particularly suitable for the fixation of the vesicles. This may be due to the additional precipitation of a chromic compound reduced from the dichromate.
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© the Japan Society of Histochemistry and Cytochemistry
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