抄録
A monoclonal antibody against rabbit kidney, γ-GTP, was prepared. The subclass of the antibody was IgG2a and the light chain was λ. The antibody specifically recognized the H chain, the site involved in anchoring part of this enzyme. The immunohistochemical assay detected reaction in the brush border of the proximal convoluted tubule of the rabbit kidney, particularly on the distal part. Cross-reaction between homologous and heterologous organs was immunohistochemically examined using this antibody. In the homologous organs, reaction was absorbed in the intercalated duct of the rabbit pancreas and along the stereocilia of the ductus epididymidis. No cross-reaction was detected in the kidney, pancreas or epididymis of a rat, or in the human kidney.
