1988 年 21 巻 6 号 p. 575-584
Intercalated duct cells and ductal basal cells in the salivary glands were suggested to be probable progenitor cells of salivary gland tumors. Different monoclonal anti-keratin antibodies (KL1, PKK1, PKK2, PKK3, RGE53, K4.62, K8.12, K8.13, K8.60, RPN1160, RPN1161, RPN1162, RPN1163, RPN1164, RPN1165, RPN1166) and polyclonal anti-keratin antiserum (TK) were tested on paraffin sections of Carnoy's- and formalin-fixed normal salivary gland tissues (parotid and submandibular glands). Monoclonal keratin antibodies could detect nos. 1, 5, 6, 7, 8, 10, 11, 13, 16, 17, 18, and 19 keratins. Intercalated duct cells of normal salivary glands displayed positive staining with monoclonal antibodies KL1, K4.62, K8.12, K8.13, K8.60, RPN1160, and RPN1164. Ductal basal cells revealed positive staining with PKK1 (nos. 19 or 18, 8) (Carnoy's fixed section), and K8.12 (nos. 16, 13), K4.62 (no. 19), RPN1165 (no. 19) keratins, which bind to nos. 19, 18, 16, 13, 8. Intercalated duct cells contained wide spectrum keratins. Keratin stainings (nos. 19 or 16, 13) of ductal basal cells in formalin fixed sections suggested nos. 19, 16, 13 proteins to be some marker for progenitor cells of salivary gland tumors.
