ANTIGEN RETRIEVAL BASED ON MICROWAVE-EXPOSURE IN IMMUNOSTAINS OF MYOSARCOMA TISSUES
1993 年 26 巻 5 号 p. 429-433
詳細
1993 年 26 巻 5 号 p. 429-433
Antigen retrieval based on microwave-exposure of formalin-fixed, paraffin-embedded tissues in a metal solution of saturated lead thiocyanate or 20% zinc sulfate was investigated. Six leiomyosarcomas and 4 rhabdomyosarcomas were studied using the ABC method applying muscle-related markers. Compared with untreated control sections, vimentin and desmin showed increased staining after the exposure. However, myoglobin immunostaining was not improved. Therefore, some tissue antigens or epitopes were retrieved that were masked by formalin-fixation, but the retrieval was not universal.
