USE OF CONFOCAL LASER SCANNING MICROSCOPE IN CYTOCHEMISTRY
1994 年 27 巻 5 号 p. 495-498
詳細
1994 年 27 巻 5 号 p. 495-498
The applications of a confocal laser scanning microscope for imaging fast dynamic changes of the intracellular calcium ion concentration ([Ca2+]i) in the living cell are described. Confocal images enable us to determine the cell-to-cell junction between clustered heart muscle cells of guinea pig and reveal that the high [Ca2+]i can evoke calcium-induced calcium release from the sarcoplasmic reticulum in the neighboring heart muscle cells through the gap junction. In rat peritoneal mast cells, secretagogue-induced calcium signaling initiates at the periphery of the cell and propagates to the nucleus as calcium wave. Nuclear [Ca2+]i is upheld at higher level than cytoplasmic [Ca2+]i for 2min. X-t scan mode with high-temporal resolution provides comprehensive images of the phenomenon. The confocal laser scanning microscope with depth-discriminating ability is a valuable tool to know a sequence of biological event within the living cell.
