抄録
Incorporated bromodeoxyuridine (BrdU) into DNA was detected in glutaraldehyde and osmium-fixed regenerating rat liver at the electron microscopic level. Without HCl hydrolysis, BrdU was localized in the nuclei of regenerating hepatocytes by post-embedding immunogold technique using the monoclonal anti-BrdU antibody on ultrathin sections after etching of the epoxy resin with 1% sodium ethoxide or 10% H2O2 followed by trypsin digestion. This procedure localized BrdU in routinely prepared specimens for electron microscopy simultaneously at a much finer structure than ordinary immunoelectron microscopy.
