抄録
The proposed combination of three histochemical methods facilitates the demonstration of microscopic anatomy, Nissl content of neurons, lipofuscin accumulation and associated cytopathological changes, if any, in one brain section. Form-aldehyde fixed paraffin sections were stained with a modified Weil's method, cresyl violet and pyronin Y in sequence, dehydrated, cleared and mounted in DPX. The slides were studied under a fluorescence microscope. The fibre system stained grey. The Nissl substance and lipofuscin stained violet and pink, respectively. On exposure to UV light, the pink stained pigment emitted golden-yellow fluorescence, resembling the auto-fluorescence of unstained lipofuscin. The final results were remarkably reproducible.
