抄録
The distribution and localization of arginase in the bovine blood cells was studied by immunofluorescent method. Specific antibody against arginase by immunizing rabbit with the corresponding antigens was conjugated with fluorescein isothiocyanate.
The fluorescence was intensely demonstrated in the reticulum cell and immature cell (erythroblast, promyelocyte, etc.) by microscopic examination of the bone marrow cells of the bovine. Slight arginase was recognized in the erythrocyte and lymphocyte, no arginase in the monocyte and polymorphonuclear neutrophilic leukocyte. Arginase distributed diffusely in the cytoplasm of above cells. It was never found in the nucleus.
Immunoproperties of fluorescent antibody was investigated by gel chromatography on Sephadex G-200 or immunoelectrophoresis. Physiologic significance of arginase in the blood cells was also discussed.
