抄録
It has been regarded almost impossible to do counter staining of tissue sections in combination with Bial's reaction, which is a direct method of demonstration of sialic acid. The reasons are; 1 reaction product which is developed in Bial's reaction is highly soluble in water and (2) since concentrated hydrochloric acid is used in Bial's reaction, the basic dyes currently used do not fit for the nuclear staining. Diezel or Ravetto, who introduced Bial's reaction into histochemistry, did not try any counter staining. In this paper, we have introduced a new method of nuclear staining which bases on the absorption of ferric ion to nuclei and the visualization of it by the formation of Prussian blue. This counter staining may be applied in combination with other histochemical procedures which should be done under a strongly acidic condition.
