The purpose is to develop floor CLT having smaller thickness and higher bending performance with two-by-four timber and stud timber than conventional CLT floor. This study reveals that the optimal CLT floor consists of two-by-four timber and stud timber as outermost layers and cross layers, respectively, on condition that each layer consists of at most 2-ply. The calculation of CLT bending performance leads to estimation results with precision. The required thickness of optimal CLT floor is about 31mm thinner than conventional CLT in average.