抄録
Two flow methods for the enzymatic determination of pyrophosphate are described that are used to diminish the consumption of reagents. One method is based on the use of an open-close circuit with manual injection using a syringe. The other is a sequential injection method. The analytical features of both methods are: a linear range of 0.4 - 20 mg L-1, an LOD of 0.38 mg L-1, and a CV of 2.0% for the sequential injection method, and a linear range of 0.3 - 15 mg L-1, an LOD of 0.29 mg L-1, and CV of 2.2% for the open-close circuit method. The methods were applied to the determination of pyrophosphate in urine. The pyrophosphate concentration determined in urine samples varied from 1.26 to 6.67 mg L-1.
