無機燐酸塩の固定について

抄録

Phosphate containing P³² was mixed with albumin and spread on an object glass. Then, before and after the action of histological fixatives the proportion of PO₄ in the spread was determined with a GEIGER-MÜLLER's counter. It was found that alcohol and acetone can prevent P³² from going out, however after washing a large amount of P³² was removed. Lead salts made insoluble precipitation with PO₄ and fixed P³² was not dissolved even by washing.
Under several fixatives which were made of formalin and metal salts one with lead salts, especially with 1% basic lead acetate had the best fixative effect in fixing PO₄.
The precipitation made of phosphate and lead acetate in a test tube was found to be very fine. The reason why a very concentrated solution of basic lead acetate had less fixative effect may possibly due to the fact that it made a finer precipitation.