補体系の病態生理に関する研究 : 第2報. 肺結核症および肝障害における補体系の動態に関する実験的研究.

抄録

(1) Complement level in experimental tuberculosis Although it has been generally believed that the formation of tuberculous cavities results from a series of allergic processes, the nature has not yet been clearly understood. The present author would like to offer the hypothesis that antigen-antibody reactions on the cellsurfaces in the lungs of animals take part in the formation of cavities as sites of complement fixation. C'1 evel was measured giving the following results, on experimental tuberculosis in guinea pigs carrying cavities produced by means of sensitization and provocation with heat-killed tubercle bacilli of H37Rv strain. 1) A transitory diminution of the serum hemolytic activity after sensitization and its persistent drop after provocation in the course of the formation of tuberculous cavities were noticed. 2) After the provocation, the drop of the serum hemolytic activity as well as the serum I A and the C'1 activities was detected. 3) More site forming units of the alveolar cell bound C'1 were detected in the groups of animals cavities with than in the control groups. 4) Fewer S.F.U of the alveolar cell bound C'1 were shown in the group of animals in which the formation of cavities was inhibited with β-methasone. The inhibitory effect of β-methasone on the formation of cavities has been clearly demonstrated by the present author. More amounts of the bound C'1 were counted in the group of animals in which no inhibitory effect of the doses of Cu-chlorophyllin administered was observed. The results above mentioned could be interpreted to show that the C'1 bound to the sites on the alvesites olar cell surfaces in the animals carrying cavities, the amounts of the bound C'1 being sufficient to fix sequen tially the following C'-components until the last step, to lead to cytolysis of the alveolar cells, and to result in the formation of tuberculous cavity.(2) C' level in experimental liver intoxication The definite correlations observed between the serum hemolytic, I A or C'1 activities and the serum transaminase levels in hepatic disorders have led the author to consider the possible roles of complement system with cytotoxic function in the course of hepatic disorders. C'1 evel were measured in the guinea pigs intoxicated with dl-ethionine. 1) Lower serum hemolytic and I A activities were detected in the experimental group than in the control group. In the experimental group, a reduced C'2 activity was regarded as a limiting factor of the low I A activity, and a lowered C'3 complex activity was as a limiting factor of the low hemolytic activity. The C'1 or C'1, 4 complex activity was not reduced at least in the experimental group. 2) The anticomplementary effect was not proved in the sera of the intoxicated animals. 3) The Tmax of EA14 prepared with the sera of the intoxicated animals was proved to be prolonged compared with that prepared with the sera of normal animals. However, both EA14 showed similar generation curves. 4) In immunolysoelectrophoretic patterns, was observed the same mobility of the serum C'1 of the experimental group as that of the control group. The mobility of the serum C'2 of the experimental group was as the same as that of the control group, Rm = 0.375, the hemolytic activity being lower in the former. 5) The hepatocyte-bound C'1 detected in the groups of animals with liver injury was as more as about three times than in the control group. It was demonstrated, from the foregoing results, that antihepatocytic antibody has been produced in the autochthonous level following intoxication with dl-ethionine. At the site on the hepatocyte membranes activated by the antibodies was detected the bound C'1 in amounts sufficient to play the cytotoxic action. No isozyme-like patterns of serum C'1 or C'2 of animals with dl-ethionine intoxication

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