A Non-radioassay of Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase by Anion-Exchange Chromatography

Koichi UEMURA; Ayako SHIMOIDE; Akiho YOKOTA

doi:10.1271/bbb.62.567

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Biological Chemistry Notes

A Non-radioassay of Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase by Anion-Exchange Chromatography

Koichi UEMURA, Ayako SHIMOIDE, Akiho YOKOTA

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Keywords: anion-exchange chromatography, assay, phosphoglycerate, ribulose 1,5-bisphosphate, ribulose 1,5-bisphosphate carboxylase/oxygenase

JOURNAL FREE ACCESS

1998 Volume 62 Issue 3 Pages 567-569

DOI https://doi.org/10.1271/bbb.62.567

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Abstract

A non-radioisotopic assay method for ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is described. 3-Phosphoglycerate produced by the RuBisCO reaction was measured using an anion-exchange chromatography. The reaction course of spinach RuBisCO measured by this method was completely the same as that measured with ¹⁴CO₂.

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