Purification and Characterization of the Overexpressed Aspergillus oryzae Xylanase, XynF1

Noriyuki KITAMOTO; Shoko YOSHINO; Kunio OHMIYA; Norihiro TSUKAGOSHI

doi:10.1271/bbb.63.1791

Microbiology & Fermentation Technology Note

Purification and Characterization of the Overexpressed Aspergillus oryzae Xylanase, XynF1

Noriyuki KITAMOTO, Shoko YOSHINO, Kunio OHMIYA, Norihiro TSUKAGOSHI

著者情報

キーワード: Aspergillus oryzae, overexpression, xylanase

ジャーナルフリー

1999 年 63 巻 10 号 p. 1791-1794

DOI https://doi.org/10.1271/bbb.63.1791

詳細

抄録

The Aspergillus oryzae xynF1 gene coding for a xylanase, XynF1, was successfully overexpressed under the strong A. oryzae TEF1 gene promoter. The high-XynF1-producing transformant secreted about 180 mg/l of XynF1 in the glucose-containing medium. The overexpressed XynF1 was purified by only one chromatographic step. The purified XynF1 had a molecular mass of 35.0 kDa, a pH optimum of 5.0, and a temperature optimum of 60°C.

責任著者(Corresponding author)

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