1999 Volume 63 Issue 5 Pages 911-916
The antigenic determinants of bovine β-casein (β-CN) were localized by using twenty overlapping peptides encompassing the entire sequence of β-CN and anti-β-CN antisera from outbred mouse, rabbit and goat. The profile of the reactions was characteristic to the species, the dominant antigenic regions being 80-95, 143-158 and 195-209 in mouse, 1-16 in rabbit and 100-115 in goat. Regions 1-16, 100-115, 121-136 and 143-158 were antigenic in all three species. The number of antigenic regions recognized by goat was much fewer than that by mouse and rabbit, possibly because of the homology between bovine and goat β-CN. A mixture of the twenty peptides could absorb about 50-60% of β-CN specific antibodies from each species. Furthermore, the mouse and rabbit anti-β-CN antibodies were also specific to the phosphorylated regions. We therefore conclude that the major antigenic determinants on β-CN would be largely sequential and include the phosphorylated sites.
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